Part:BBa_K5250011:Design

constitutive promoter + XutR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 100
  Illegal BamHI site found at 1236
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 669
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The basic parts in this composite parts have been codon optimized.

Source

We are using the promoter sequence from the iGEM Part registry. The sequence for XutR we took from the following paper: https://doi.org/10.1128/aem.00250-20